Ukrainian paratroopers have been defending the airport since April 2014 in complete surroundings without ground connection. Ukrainian in July the troops made their way to the airfield, unblocking it.

A series of battles in 2014 for control of the city of Lyman (formerly Krasny Liman) of the Donetsk region during the war in eastern Ukraine. Fortifications of Russian militants were destroyed in the southern part Krasnolymansky district (in the city of Siversk, the villages of Yampil and Zarichne, the village of Zakitne and the remains in the city of Krasny Liman). During the raid part of the gang formation of criminals was detained and brought to criminal liability. However, most of them are Russian militants redeployed to the city of Sloviansk.

Defense of the Kramatorsk checkpoint: June 9, 2014, despite against difficulties and losses, the brigade successfully defended the strategic post.

On July 5, 2014, the fighters of the brigade participated in repelling the attack columns of heavy equipment of militants at the 5th checkpoint near Sloviansk. As a result of the battle, a fighter of the Mendel Roman brigade died, the attack was on repulsed, the terrorists' equipment was burned. On the same day, the fighters of the brigade together with other formations entered Slovyansk.

Taking control of strategic settlements: In August 2014 year, the brigade was involved in takeover operations Vugleghirsk, Oleksandrivskogo, Vilkhuvatka, Bulavynskogo.

In the winter campaign of 2015, the fighters of the brigade took part in battles for Donetsk Airport, Avdiivka. The personnel took part in carrying out an anti-terrorist operation in the area of populated areas Avdiivka, Toretsk, Zaitseve, Mayorske, Shchastya, Stanytsia-Luhansk.

Battles for the Donetsk airport: In January 2015, part of the brigade was in the battle zone for this key object.

Continued participation in hostilities in 2019: The brigade withstood the positions in Luhansk region for almost seven months, attracting about 1000 soldiers.

A full-scale invasion was met by units of the brigade on Avdiivsk direction, where the defense was held until June 2022.

The liberation of the city of Izyum in September 2022 was one of the important ones operations during the counteroffensive of Ukrainian troops in Kharkiv region during the Russian-Ukrainian war. 
 The role of the 25th separate of the Sicheslav Airborne Brigade in the liberation of the city of Izyum was key to the successful recapture of the territory from Russia occupation On the eve of the liberation of Izyum, fighters of the 25th separate of the Sicheslav Airborne Brigade were among the first to broke through the corridor to the city of Izyum, which was under control at that time Russian troops. 
 Through timely coordination with others military formations, the 25th separate brigade worked in concerted action with other military units and units that also took part in the liberation of the city and entered it with other parties.

In the period from October 2022 to June 2023, the personnel of the brigade participated in the conduct of the defense operation on Swativsko-Kreminsk direction. 
 Personal since July 2023 brigade performs tasks in the Kupyansk-Svativsk direction, holding back the enemy's daily assaults.